reversed-phase c 4 column protein c 4  (Grace Vydac Inc)

Journal: eLife

Article Title: The ER folding sensor UGGT1 acts on TAPBPR-chaperoned peptide-free MHC I

doi: 10.7554/eLife.85432

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Article Snippet: Peptides bound to pMHC I molecules were analyzed using an ACQUITY UPLC Protein BEH C 4 column (Waters). pMHC I molecules were on-column dissociated.

Techniques: Construct, Recombinant, Plasmid Preparation, Expressing, Software, Purification, Protein Purification, Virus

Journal: Biochemistry

Article Title: 18 F-Labeled Insulin: A Prosthetic Group Methodology for Incorporation of a Positron Emitter into Peptides and Proteins †

doi:

Figure Lengend Snippet: Purification of [18F]FMB-insulin ([18F]-4b) using RP-HPLC. The peak corresponding to [18F]-4b is denoted by the arrow. For conditions, see Materials and Methods.

Article Snippet: The total mixture was injected onto a C-4 protein analytical HPLC column (Vydac, 214TP, 4.6 mm × 250 mm) and eluted with 32% aqueous acetonitrile containing 0.1% TFA at a rate of 0.9 mL/min.

Techniques: Purification

Journal: Biochemistry

Article Title: 18 F-Labeled Insulin: A Prosthetic Group Methodology for Incorporation of a Positron Emitter into Peptides and Proteins †

doi:

Figure Lengend Snippet: Specific binding of 18F-labeled insulin to insulin receptors. In two separate experiments IM-9 cells were incubated for 75 min with 18F-labeled insulin (○) or 125I-labeled insulin (▲) over a range of concentrations from 0.01 to 9 ng/mL under conditions described in the legend for Figure 3. The concentrations of 18F-labeled insulin used were determined by radioimmunoassay and by UV absorbance at 208 nm of concentrated solution using RP-HPLC as described under Materials and Methods. The difference in bound to free labeled hormone (B/F) for the two insulin derivatives in part reflects differences in cell numbers.

Article Snippet: The total mixture was injected onto a C-4 protein analytical HPLC column (Vydac, 214TP, 4.6 mm × 250 mm) and eluted with 32% aqueous acetonitrile containing 0.1% TFA at a rate of 0.9 mL/min.

Techniques: Binding Assay, Labeling, Incubation, RIA Assay